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Determination of Genetic Diversity among Local Mandarin Oranges (Citrus reticulata) Using Mature Seed Derived Calli through RAPD Markers

Corresponding Author : Hammadul Hoque (

Authors : Beethi Sinha , Md. Muzahidul Islam , Fahmid H. Bhuiyan , Md. Nazmul Hasan , Mohammad Golam Kibria , Rabeya Sultana , Shamsul H. Prodhan , Hammadul Hoque

Keywords : Diversity, UPGMA, PIC, RAPD, Dendrogram

Abstract :

Orange (Citrus reticulata) is cultivated at several districts of Bangladesh but the genetic diversity among cultivars is still unknown. This research aimed to find out the diversity among locally grown nine mandarin orange (C. reticulata) cultivars. For this purpose, calli were initiated and used as a source for genomic DNA. Murashige and Skoog (MS) medium supplemented with phytohormones (2,4-dichlorophenoxyacetic acid, benzyl adenine, 1-naphthalene acetic acid, kinetin, indole-3-butyric acid) and malt extract were used for calli induction. DNA extracted from the calli of all the test cultivars’ further subjected to PCR with ten RAPD markers, subsequently, data were assayed employing AlphaEaseFc 4.0 software, UPGMA (Unweighted Pair Group Method of Arithmetic Means), and the dendrogram was built by Statistica 7. The highest polymorphism information content (PIC) value was 0.79 for decamer OPX 04, and the lowest PIC value was 0.49 for both OPB 04 and OPB 17. From this study, the inter-relationship between nine cultivars was observed where Beanibazar, Bandarban, and Tamabil cultivars were closely related, followed by Jaflong and Karimganj cultivars. Companiganj and Darjeeling cultivars also shared some degree of genetic makeup. This study concludes that the used markers could be not only efficiently utilized in diversity analysis among C. reticulata cultivars but also for further improvement of local mandarin orange.

Published on December 31st, 2022 in Volume 32, Issue 1, Life Sciences